使用亲脂性阳离子测量燕麦叶片原生质体的膜电位。
Use of lipophilic cations to measure the membrane potential of oat leaf protoplasts.
作者信息
Rubinstein B
机构信息
Department of Botany, University of Massachusetts, Amherst, Massachusetts 01003.
出版信息
Plant Physiol. 1978 Dec;62(6):927-9. doi: 10.1104/pp.62.6.927.
Abstract
Uptake of the lipophilic cation triphenylmethylphosphonium into mesophyll protoplasts of oat (Avena sativa L. cv. "Garry") approaches equilibrium at 3 to 4 hours. The resulting external and internal concentrations are then used with the Nernst equation to obtain a membrane potential of -62 millivolts, inside negative. Potentials calculated in this manner are depolarized by adding 2 mm sodium azide and 50 mum carbonyl cyanide m-chlorophenylhydrazone as well as by increasing the external proton and potassium concentrations. The depolarizations are qualitatively similar to those seen when oat mesoyphll cells are measured in situ with microelectrodes. It is concluded that due to the lack of turgor and fragility of protoplasts, estimations of their membrane potential may be made more reliably, under some conditions, with lipophilic cations than with microelectrodes.
摘要
亲脂性阳离子三苯基甲基鏻进入燕麦（Avena sativa L. cv. “Garry”）叶肉原生质体的过程在3至4小时接近平衡。然后将所得的外部和内部浓度与能斯特方程一起用于获得-62毫伏的膜电位，内部为负。通过添加2 mM叠氮化钠和50 μM羰基氰化物间氯苯腙以及增加外部质子和钾浓度，以这种方式计算出的电位会发生去极化。这些去极化在定性上与用微电极原位测量燕麦叶肉细胞时观察到的相似。得出的结论是，由于原生质体缺乏膨压且易碎，在某些条件下，与微电极相比，用亲脂性阳离子更可靠地估计其膜电位。
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