蔗糖转运至发育中大豆子叶原生质体的能量学
Energetics of sucrose transport into protoplasts from developing soybean cotyledons.
作者信息
Lin W
机构信息
Central Research and Development Department, E. I. du Pont de Nemours and Company, Experimental Station, Wilmington, Delaware 19898.
出版信息
Plant Physiol. 1985 May;78(1):41-5. doi: 10.1104/pp.78.1.41.
Abstract
The accumulation of tetraphenylphosphonium (TPP(+)), 5,5'-dimethyl-oxazolidine-2,4-dione (DMO), and a micro pH electrode were used to measure membrane potential, intracellular and extracellular pH, respectively, upon the addition of exogenous sucrose to soybean cotyledon protoplasts. Addition of sucrose caused a specific and transient (a) depolarization of the membrane potential (measured by TPP(+) accumulation), (b) acidification of the intracellular pH (measured by DMO accumulation), and (c) alkalization of the external medium (measured by a micro pH electrode). The time course for all these changes was similar (i.e. 5 to 10 minutes). Based on the rate of sucrose uptake and alkalization of the external medium, a stoichiometry of 1.02 to 1.10 for proton to sucrose was estimated. These data strongly support a proton/sucrose cotransporting mechanism in soybean cotyledon cells.
摘要
向大豆子叶原生质体添加外源蔗糖后，利用四苯基鏻（TPP(+)）、5,5'-二甲基恶唑烷-2,4-二酮（DMO）的积累以及微型pH电极分别测量膜电位、细胞内和细胞外pH。添加蔗糖导致：（a）膜电位出现特异性瞬时去极化（通过TPP(+)积累测量），（b）细胞内pH酸化（通过DMO积累测量），以及（c）外部介质碱化（通过微型pH电极测量）。所有这些变化的时间进程相似（即5至10分钟）。根据蔗糖摄取速率和外部介质碱化情况，估计质子与蔗糖的化学计量比为1.02至1.10。这些数据有力地支持了大豆子叶细胞中质子/蔗糖共转运机制。
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本文引用的文献
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2
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