2,4-二羟基-7-甲氧基-2H-1,4-苯并恶嗪-3(4H)-酮，一种来自玉米的抑制剂，对软腐欧文氏菌属具有不同的活性。
2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, an Inhibitor from Zea mays with Differential Activity against Soft Rotting Erwinia Species.
机构信息
Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706.
出版信息
Plant Physiol. 1978 May;61(5):791-5. doi: 10.1104/pp.61.5.791.
[2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one] DIMBOA was extracted with ethyl acetate from acidified water homogenates of corn (Zea mays L.) seedlings. Pure DIMBOA or ethyl acetate extracts of corn tissue were added to bacterial growth medium at five concentrations (measured as hydroxamates). DIMBOA and corn extracts were more inhibitory to soft rot bacteria (Erwinia spp.) that are nonpathogenic to corn than to soft rot bacteria that are corn pathogens. The inhibitory activity of DIMBOA was similar to that of the ethyl acetate extracts. Both corn extracts and DIMBOA prolonged the lag phase of bacterial growth without significantly changing log phase growth rates. At various concentrations of the inhibitor, 50 to 100% of the activity of corn extracts inhibitory to different bacterial isolates was attributable to DIMBOA. Extracts of DIMBOA-deficient plants (genotype bxbx) were not inhibitory to Erwinia spp. It was concluded that DIMBOA is the major active component in those corn extracts which are inhibitory to soft rot Erwinia species.
[2,4-二羟基-7-甲氧基-2H-1,4-苯并恶嗪-3(4H)-酮] DIMBOA 可从酸化水匀浆的玉米（Zea mays L.）幼苗中用乙酸乙酯提取。纯 DIMBOA 或玉米组织的乙酸乙酯提取物以五个浓度（以羟肟酸盐计）添加到细菌生长培养基中。与对玉米致病的软腐病菌相比，对玉米非致病的软腐病菌，DIMBOA 和玉米提取物的抑制作用更强。DIMBOA 的抑制活性与乙酸乙酯提取物相似。玉米提取物和 DIMBOA 均延长了细菌生长的迟滞期，而对数生长期的生长速率没有明显变化。在抑制剂的各种浓度下，不同细菌分离物的玉米提取物的 50%至 100%的抑制活性归因于 DIMBOA。缺乏 DIMBOA 的植物（基因型 bxbx）的提取物对欧文氏菌属没有抑制作用。结论是，在对软腐欧文氏菌属具有抑制作用的那些玉米提取物中，DIMBOA 是主要的活性成分。
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