Bartz J, Söll D, Burrows W J, Skoog F
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1448-53. doi: 10.1073/pnas.67.3.1448.
The distribution of the two cytokinin-active ribonucleosides 6-(3-methyl-2-butenylamino)-9-beta-D-ribofuranosylpurine (1) and 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine (2) in tRNA(Phe), tRNA(UUG) (Leu), tRNA(UCR) (Ser), tRNA(Tyr), tRNA(Cys), and tRNA(2) (Trp) from stationary phase Escherichia coli has been studied. Compound 2 predominated in all these species except tRNA(Phe), in which compound 1 was greatly in excess. Compound 1 was present in relatively small amounts in tRNA(UUG) (Leu), tRNA(Cys), and tRNA(2) (Trp), and was not detected in tRNA(UCR) (Ser) and tRNA(Tyr). Cells from the early logarithmic phase had the highest cytokinin content in their tRNA. Stationary phase tRNA had a slight increase in the amount of 1, which may be explained by the appearance of the new tRNA(Phe). No differences were observed between the chromatographic patterns of tRNAs from early-, mid-, and latelogarithmic phases.
已对来自稳定期大肠杆菌的tRNA（苯丙氨酸）、tRNA（UUG）（亮氨酸）、tRNA（UCR）（丝氨酸）、tRNA（酪氨酸）、tRNA（半胱氨酸）和tRNA（2）（色氨酸）中两种具有细胞分裂素活性的核糖核苷6-（3-甲基-2-丁烯基氨基）-9-β-D-呋喃核糖基嘌呤（1）和6-（3-甲基-2-丁烯基氨基）-2-甲硫基-9-β-D-呋喃核糖基嘌呤（2）的分布进行了研究。除tRNA（苯丙氨酸）中化合物1大量过剩外，化合物2在所有这些种类中占主导地位。化合物1在tRNA（UUG）（亮氨酸）、tRNA（半胱氨酸）和tRNA（2）（色氨酸）中的含量相对较少，在tRNA（UCR）（丝氨酸）和tRNA（酪氨酸）中未检测到。对数期早期细胞的tRNA中细胞分裂素含量最高。稳定期tRNA中1的含量略有增加，这可能是由于新的tRNA（苯丙氨酸）出现所致。对数期早期、中期和晚期tRNA的色谱图之间未观察到差异。
