分析苋菜花青素测定细胞分裂素的变异性：离体子叶“老化”的影响。
Analysis of variability in the amaranthus betacyanin assay for cytokinins: effects of "aging" excised cotyledons.
机构信息
School of Biological Sciences, Flinders University of South Australia, Bedford Park, South Australia 5042.
出版信息
Plant Physiol. 1979 Feb;63(2):274-6. doi: 10.1104/pp.63.2.274.
"Aging" of excised cotyledons plus the top part of the hypocotyl of Amaranthus tricolor seedlings was carried out by washing in distilled H(2)O for varying periods. This led to increased betacyanin accumulation during the subsequent 24-hour induction period in the presence of tyrosine and Na(+) + K(+) phosphate. Endogenous accumulation as well as that dependent on added benzyladenine and on added fusicoccin was stimulated. This stimulation could not be due to a carryover of a wound-induced burst of ethylene since 2-chloroethylphosphonic acid (Ethrel) was shown to be extremely inhibitory to betacyanin synthesis if present during the induction process. It is possible that a wound-induced burst of ethylene could give rise to increased betacyanin synthesis as an after effect. The procedure for obtaining good induction with the most reproducible results is described.
将苋菜幼苗的离体子叶和下胚轴的顶部在蒸馏水中洗涤不同时间，进行“老化”处理。这导致在随后的 24 小时诱导期内，在酪氨酸和 Na(+) + K(+)磷酸盐存在的情况下，甜菜红素的积累增加。内源积累以及依赖于添加的苄基腺嘌呤和添加的 fusicoccin 的积累都受到刺激。这种刺激不可能是由于乙烯诱导的爆发的残留，因为 2-氯乙基膦酸（乙烯利）如果在诱导过程中存在，对甜菜红素合成具有极强的抑制作用。有可能是乙烯诱导的爆发会导致甜菜红素合成的增加作为一种后效应。描述了获得良好诱导和最可重复结果的程序。
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