用香草酸使夜光藻生物发光的昼夜节律相位移动。
Shifting the phase of the circadian rhythm in bioluminescence in gonyaulax with vanillic Acid.
机构信息
Department of Biological Sciences and College of Creative Studies, University of California, Santa Barbara, California 93106.
出版信息
Plant Physiol. 1979 Feb;63(2):324-7. doi: 10.1104/pp.63.2.324.
Abstract
Exposure for 4 hours to vanillic acid (4-hydroxy 3-methoxy benzoic acid) caused large delay phase shifts (5 to 6 hours) in the circadian rhythm of bioluminescence in Gonyaulax polyedra, when assayed at either 10 to 14 circadian time or 22 to 02 circadian time in constant light and temperature, provided that the pH of the medium was 7.1 or lower. Corresponding changes in the pH with acetic acid did not shift phase. Vanillic acid caused detectable depolarization of the membranes of Gonyaulax, as demonstrated with the cyanine dye fluorescence technique.
摘要
暴露在香草酸（4-羟基-3-甲氧基苯甲酸）中 4 小时，会导致 Gonyaulax polyedra 生物发光的昼夜节律出现大的延迟相位移动（5 到 6 小时），无论是在恒定光照和温度下的 10 到 14 昼夜时间还是 22 到 02 昼夜时间进行测定，如果介质的 pH 值为 7.1 或更低。用乙酸引起的 pH 值的相应变化不会改变相位。香草酸会导致 Gonyaulax 细胞膜可检测到去极化，这可以用花青染料荧光技术证明。
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