Department of Botany and Zoology, Massey University, Palmerston North, New Zealand.
Plant Physiol. 1974 Dec;54(6):855-8. doi: 10.1104/pp.54.6.855.
The membrane potentials of aged, excised barley (Hordeum vulgare L.) root cells were rapidly depolarized by the addition of salicylic acid (o-hydroxybenzoic acid) to the buffered medium bathing root segments. Initial values for membrane potentials were restored very slowly (within 100 minutes) by replacing the phenolic solution by phenolic-free buffer. Several other naturally occurring benzoic and cinnamic acids depolarized cell membrane potentials. The cinnamic acids consistently caused a greater depolarization than the correspondingly substituted benzoic acids. A strong positive correlation was found between the depolarization values (DeltaE) for the benzoic acids and their lipid solubilities. This study supports the hypothesis that the inhibition of ion uptake brought about by naturally occurring phenolic acids is caused by a generalized increase in membrane permeability to inorganic ions.
用缓冲液浸浴大麦(Hordeum vulgare L.)根段,外加柳酸(邻羟基苯甲酸)可使老龄离体大麦根细胞的膜电位迅速去极化。用不含酚的缓冲液替换含酚溶液后,膜电位的初始值恢复得非常缓慢(100 分钟内)。几种其他天然存在的苯甲酸和肉桂酸也使细胞膜电位去极化。肉桂酸比相应取代的苯甲酸引起更大的去极化。发现苯甲酸的去极化值(ΔE)与其脂溶性之间存在很强的正相关。这项研究支持这样一种假说,即天然存在的酚酸引起的离子摄取抑制是由于无机离子透过细胞膜的通透性普遍增加所致。