Young P A, Kaiser I I
Departments of Biochemistry and Chemistry, University of Wyoming, Laramie, Wyoming 82071.
Plant Physiol. 1979 Mar;63(3):511-7. doi: 10.1104/pp.63.3.511.
A procedure has been developed for the isolation of transfer RNA from the selenium accumulator plant Astragalus bisulcatus. This material appears free of interfering phenolic compounds, has a high guanosine to cytidine ratio, shows a major and modified nucleoside composition characteristic of plant transfer RNAs, and exhibits chromatographic and electrophoretic properties similar to transfer RNAs from other well studied bacterial and plant systems. RNAs isolated from A. bisulcatus seedlings incubated in the presence of (75)Se indicate some incorporation of radioactivity into the transfer RNAs, but at extremely low levels. The transfer RNAs were active in accepting amino acids, although their over-all levels of activity appeared low when compared with those from a homologous Escherichia coli aminoacylation reaction system.
已开发出一种从硒富集植物双槽黄芪中分离转运RNA的方法。该材料似乎不含干扰性酚类化合物，鸟苷与胞苷的比例较高，具有植物转运RNA特有的主要和修饰核苷组成，并且具有与其他经过充分研究的细菌和植物系统中的转运RNA相似的色谱和电泳特性。在（75）硒存在下培养的双槽黄芪幼苗中分离出的RNA表明，有一些放射性掺入到转运RNA中，但水平极低。这些转运RNA在接受氨基酸方面具有活性，尽管与同源大肠杆菌氨酰化反应系统相比，它们的总体活性水平似乎较低。
