用于检测猫白血病病毒和猫免疫缺陷病毒的酶联免疫吸附测定方法。
Enzyme-linked immunosorbent assay methods for detection of feline leukemia virus and feline immunodeficiency virus.
作者信息
Tonelli Q J
机构信息
IDEXX Corporation, Westbrook, ME 04092.
出版信息
J Am Vet Med Assoc. 1991 Nov 15;199(10):1336-9.
Enzyme-linked immunosorbent assays have been widely used for diagnosis of FeLV and feline immunodeficiency virus (FIV) infections. Various ELISA kits for FeLV are available from several manufacturers. Although these tests are configured in a variety of formats, they are all direct antigen-detection systems for the viral core protein p27. On the other hand, ELISA for FIV exposure detects specific feline antibody to FIV. Basic immunoassay principles and the application of ELISA technology used in FeLV and FIV ELISA kits are described.
酶联免疫吸附测定已广泛用于猫白血病病毒（FeLV）和猫免疫缺陷病毒（FIV）感染的诊断。有几家制造商提供多种用于检测FeLV的酶联免疫吸附测定试剂盒。尽管这些检测采用了多种形式，但它们都是用于检测病毒核心蛋白p27的直接抗原检测系统。另一方面，用于检测FIV暴露情况的酶联免疫吸附测定检测的是针对FIV的特异性猫抗体。本文描述了基本免疫测定原理以及FeLV和FIV酶联免疫吸附测定试剂盒中使用的酶联免疫吸附测定技术的应用。
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