利用蛋白质印迹法和放射免疫沉淀法诊断猫白血病和猫免疫缺陷病毒感染。
Use of western blot and radioimmunoprecipitation for diagnosis of feline leukemia and feline immunodeficiency virus infections.
作者信息
Egberink H F, Lutz H, Horzinek M C
机构信息
Department of Infectious Diseases and Immunology, Veterinary Faculty, Institute of Virology, Utrecht, The Netherlands.
出版信息
J Am Vet Med Assoc. 1991 Nov 15;199(10):1339-42.
The uses and limitations of the western blot (WB) and radioimmunoprecipitation assay (RIPA) techniques for study of feline immunodeficiency virus (FIV) and FeLV were evaluated. Western blot analysis was used to detect antigenic relatedness between the 2 lentiviruses. Using a rabbit serum directed against p26 of the equine infectious anemia virus (EIAV) and anti-EIAV horse serum obtained from an infected horse, cross-reactivity with p24 of FIV was revealed. Cat sera obtained late after experimentally induced FIV infection recognized p26 of EIAV, which indicates reciprocal cross-reactivity. For RIPA, FIV was metabolically labeled, and virus-coded proteins were identified, using immunoprecipitation. Polypeptides with apparent molecular mass of about 15, 24, 43, 50, 120, and 160 kilodaltons were detected. An additional polypeptide of 10 kilodaltons was found only by use of WB analysis.
评估了蛋白质免疫印迹法（WB）和放射免疫沉淀法（RIPA）技术在研究猫免疫缺陷病毒（FIV）和猫白血病病毒（FeLV）方面的用途及局限性。蛋白质免疫印迹分析用于检测这两种慢病毒之间的抗原相关性。使用针对马传染性贫血病毒（EIAV）p26的兔血清以及从感染马获得的抗EIAV马血清，发现与FIV的p24存在交叉反应。实验性诱导FIV感染后晚期获得的猫血清可识别EIAV的p26，这表明存在相互交叉反应。对于放射免疫沉淀法，对FIV进行代谢标记，并通过免疫沉淀鉴定病毒编码的蛋白质。检测到表观分子量约为15、24、43、50、120和160千道尔顿的多肽。仅通过蛋白质免疫印迹分析发现了一条额外的10千道尔顿的多肽。
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