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1
Derepression and Repression of Lysine-sensitive Aspartokinase during in Vitro Culture of Carrot Root Tissue.胡萝卜根组织体外培养过程中赖氨酸敏感天冬氨酸激酶的去阻遏和阻遏。
Plant Physiol. 1979 Mar;63(3):583-5. doi: 10.1104/pp.63.3.583.
2
Change in the proportion of two aspartokinases in carrot root tissue in response to in vitro culture.胡萝卜根组织中两种天冬氨酸激酶比例的变化对离体培养的响应。
Plant Physiol. 1978 Jan;61(1):115-8. doi: 10.1104/pp.61.1.115.
3
Regulation of lysine and threonine synthesis in carrot cell suspension cultures and whole carrot roots.胡萝卜细胞悬浮培养物和完整胡萝卜根中天冬氨酸和苏氨酸合成的调控。
Planta. 1978 Jan;141(3):315-21. doi: 10.1007/BF00388350.
4
Expression of aspartokinase, dihydrodipicolinic acid synthase and homoserine dehydrogenase during growth of carrot cell suspension cultures on lysine- and threonine-supplemented media.在补充赖氨酸和苏氨酸的培养基上胡萝卜细胞悬浮培养物生长过程中天冬氨酸激酶、二氢吡啶二羧酸合酶和高丝氨酸脱氢酶的表达。
Z Naturforsch C Biosci. 1979 Dec;34(12):1177-85. doi: 10.1515/znc-1979-1216.
5
Identification and expression of a cDNA from Daucus carota encoding a bifunctional aspartokinase-homoserine dehydrogenase.来自胡萝卜的一个编码双功能天冬氨酸激酶-高丝氨酸脱氢酶的cDNA的鉴定与表达。
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6
Regulation of lysine- and lysine-plus-threonine-inhibitable aspartokinases in Bacillus brevis.短短芽孢杆菌中赖氨酸及赖氨酸加苏氨酸可抑制的天冬氨酸激酶的调控
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7
Participation of lysine-sensitive aspartokinase in threonine production by S-2-aminoethyl cysteine-resistant mutants of Serratia marcescens.赖氨酸敏感天冬氨酸激酶在粘质沙雷氏菌S-2-氨基乙基半胱氨酸抗性突变体苏氨酸生产中的作用
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Bifunctional protein in carrot contains both aspartokinase and homoserine dehydrogenase activities.胡萝卜中的双功能蛋白同时具有天冬氨酸激酶和高丝氨酸脱氢酶的活性。
Plant Physiol. 1991 Dec;97(4):1323-8. doi: 10.1104/pp.97.4.1323.
10
Regulatory isoenzymes of aspartate kinase and the control of lysine and threonine biosynthesis in carrot cell suspension culture.天冬氨酸激酶的调节同工酶与胡萝卜细胞悬浮培养中天冬氨酸、赖氨酸和苏氨酸生物合成的调控。
Plant Physiol. 1978 Oct;62(4):536-41. doi: 10.1104/pp.62.4.536.

引用本文的文献

1
Bifunctional protein in carrot contains both aspartokinase and homoserine dehydrogenase activities.胡萝卜中的双功能蛋白同时具有天冬氨酸激酶和高丝氨酸脱氢酶的活性。
Plant Physiol. 1991 Dec;97(4):1323-8. doi: 10.1104/pp.97.4.1323.
2
Methionine Biosynthesis in Lemna: STUDIES ON THE REGULATION OF CYSTATHIONINE gamma-SYNTHASE, O-PHOSPHOHOMOSERINE SULFHYDRYLASE, AND O-ACETYLSERINE SULFHYDRYLASE.浮萍中的甲硫氨酸生物合成：胱硫醚γ-合酶、O-磷酸高丝氨酸巯基酶和O-乙酰丝氨酸巯基酶的调控研究
Plant Physiol. 1982 May;69(5):1077-83. doi: 10.1104/pp.69.5.1077.

本文引用的文献

1
Change in the proportion of two aspartokinases in carrot root tissue in response to in vitro culture.胡萝卜根组织中两种天冬氨酸激酶比例的变化对离体培养的响应。
Plant Physiol. 1978 Jan;61(1):115-8. doi: 10.1104/pp.61.1.115.
2
Properties and Regulation of Aspartate Kinase from Barley Seedlings (Hordeum vulgare L.).大麦幼苗（Hordeum vulgare L.）天冬氨酸激酶的性质及其调控。
Plant Physiol. 1977 Jan;59(1):69-73. doi: 10.1104/pp.59.1.69.
3
Aspartokinase from wheat germ: isolation, characterization, and regulation.来自小麦胚芽的天冬氨酸激酶：分离、特性及调控
Plant Physiol. 1973 Feb;51(2):322-6. doi: 10.1104/pp.51.2.322.
4
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
5
Multivalent repression in the biosynthesis of threonine in Salmonella typhimurium and Escherichia coli.鼠伤寒沙门氏菌和大肠杆菌中苏氨酸生物合成的多价阻遏
Biochem Biophys Res Commun. 1963 Feb 6;10:277-82. doi: 10.1016/0006-291x(63)90430-3.
6
Regulation of the nitrate assimilation pathway of cultured tobacco cells. II. Properties of a variant cell line.培养烟草细胞硝酸盐同化途径的调控。II. 一个变异细胞系的特性
Biochim Biophys Acta. 1970 Jul 21;215(1):152-65. doi: 10.1016/0304-4165(70)90398-3.
7
Isolation and characterization of a lysine-sensitive aspartokinase from a multicellular plant.从一种多细胞植物中分离并鉴定一种赖氨酸敏感型天冬氨酸激酶
Biochem Biophys Res Commun. 1970 Dec 9;41(5):1211-7. doi: 10.1016/0006-291x(70)90215-9.

胡萝卜根组织体外培养过程中赖氨酸敏感天冬氨酸激酶的去阻遏和阻遏。

Derepression and Repression of Lysine-sensitive Aspartokinase during in Vitro Culture of Carrot Root Tissue.

机构信息

Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan.

出版信息

Plant Physiol. 1979 Mar;63(3):583-5. doi: 10.1104/pp.63.3.583.

DOI:10.1104/pp.63.3.583
PMID:16660771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC542874/
Abstract

The increment of lysine-sensitive aspartokinase (EC 2.7.2.4) activity during in vitro culture of carrot (Daucus carota, cv. Oogata sanzun) root tissue was explained in terms of derepression caused by an earlier decrease in the endogenous level of lysine, a possible end product repressor. Tissue content of free lysine decreased to about one-third of the initial level after 1 day of culture and no lysine was detected in the 2nd day. Inclusion of lysine (0.1 to 1.0 millimolar) in the culture medium resulted in a specific suppression of increase in lysine-sensitive aspartokinase activity without affecting the increase in threonine-sensitive aspartokinase activity.

摘要

在体外培养胡萝卜（Daucus carota，cv. Oogata sanzun）根组织期间，赖氨酸敏感型天冬氨酸激酶（EC 2.7.2.4）活性的增加可以用赖氨酸水平的早期降低引起的去阻遏来解释，赖氨酸可能是一种终产物阻遏物。在培养的第 1 天，组织中游离赖氨酸的含量下降到初始水平的约三分之一，第 2 天未检测到赖氨酸。在培养基中添加赖氨酸（0.1 至 1.0 毫摩尔）可特异性抑制赖氨酸敏感型天冬氨酸激酶活性的增加，而不影响苏氨酸敏感型天冬氨酸激酶活性的增加。