胡萝卜根组织中两种天冬氨酸激酶比例的变化对离体培养的响应。
Change in the proportion of two aspartokinases in carrot root tissue in response to in vitro culture.
机构信息
Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo, Japan.
出版信息
Plant Physiol. 1978 Jan;61(1):115-8. doi: 10.1104/pp.61.1.115.
Abstract
Two isofunctional aspartokinases (EC 2.7.2.4) exist in fresh root tissue of carrot (Daucus carota, cv. Oogata sanzun). The threoninesensitive portion constitutes about 70% of the activity; the lysinesensitive, less than 20%. Culture of slices of carrot tissue for 3 days reversed the ratio as the lysine-sensitive activity preferentially increased. Inhibition by threonine and lysine was additive in both enzyme preparations from fresh and cultured tissues. The activities were resolved into two distinct fractions of different sensitivity to threonine and lysine by DEAE-Sephadex A-50 column chromatography.
摘要
新鲜胡萝卜（Daucus carota，cv. Oogata sanzun）根组织中存在两种同工型天冬氨酸激酶（EC 2.7.2.4）。苏氨酸敏感部分约占活性的 70%；赖氨酸敏感部分小于 20%。胡萝卜组织切片培养 3 天后，这种比例发生逆转，因为赖氨酸敏感活性优先增加。两种酶制剂均来自新鲜和培养组织，苏氨酸和赖氨酸的抑制作用具有加性。DEAE-Sephadex A-50 柱层析将活性分成两个不同的分数，对苏氨酸和赖氨酸的敏感性不同。
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