天冬氨酸激酶的调节同工酶与胡萝卜细胞悬浮培养中天冬氨酸、赖氨酸和苏氨酸生物合成的调控。
Regulatory isoenzymes of aspartate kinase and the control of lysine and threonine biosynthesis in carrot cell suspension culture.
机构信息
Biochemistry Department, Rothamsted Experimental Station, Harpenden, Hertsfordshire AL5 2JQ United Kingdom.
出版信息
Plant Physiol. 1978 Oct;62(4):536-41. doi: 10.1104/pp.62.4.536.
Abstract
Aspartate kinase (EC 2.7.2.4) from carrot (Daucus carota L.) cell suspension culture has been partially resolved into lysine-sensitive and threonine-sensitive components by gel filtration chromatography. The yield of lysine-sensitive aspartate kinase changed independently of the yield of the threonine-sensitive activity during the 4-week growth cycle of the culture, and this provides additional evidence for the existence of two independently regulated isoenzymes. Exogenously supplied lysine and threonine specifically inhibited the in vivo formation of lysine and threonine, respectively, from radioactive acetate.
摘要
从胡萝卜（Daucus carota L.）细胞悬浮培养物中分离出的天冬氨酸激酶（EC 2.7.2.4）通过凝胶过滤色谱法部分分离为赖氨酸敏感和苏氨酸敏感的组分。在培养物的 4 周生长周期中，赖氨酸敏感的天冬氨酸激酶的产率与苏氨酸敏感活性的产率独立变化，这为存在两种独立调节的同工酶提供了额外的证据。外源性提供的赖氨酸和苏氨酸分别特异性地抑制了从放射性乙酸中体内形成赖氨酸和苏氨酸。
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本文引用的文献
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Properties and Regulation of Aspartate Kinase from Barley Seedlings (Hordeum vulgare L.).大麦幼苗（Hordeum vulgare L.）天冬氨酸激酶的性质及其调控。
Plant Physiol. 1977 Jan;59(1):69-73. doi: 10.1104/pp.59.1.69.
2
Changes in Enzyme Regulation during Growth of Maize: II. Relationships among Multiple Molecular Forms of Homoserine Dehydrogenase.玉米生长过程中酶调节的变化：II. 同型丝氨酸脱氢酶多种分子形式之间的关系。
Plant Physiol. 1975 Jun;55(6):999-1005. doi: 10.1104/pp.55.6.999.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Isolation and characterization of a lysine-sensitive aspartokinase from a multicellular plant.从一种多细胞植物中分离并鉴定一种赖氨酸敏感型天冬氨酸激酶
Biochem Biophys Res Commun. 1970 Dec 9;41(5):1211-7. doi: 10.1016/0006-291x(70)90215-9.
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Studies on the catalytic and regulatory properties of homoserine dehydrogenase of Zea mays roots.玉米根中高丝氨酸脱氢酶的催化和调节特性研究。
Biochim Biophys Acta. 1969 Feb 11;171(2):205-16. doi: 10.1016/0005-2744(69)90154-5.
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Tritosol: a new scintillation cocktail based on Triton X-100.三乙醇胺：一种基于曲拉通X-100的新型闪烁液。
Anal Biochem. 1975 Feb;63(2):555-8. doi: 10.1016/0003-2697(75)90379-6.
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The enzymology of lysine biosynthesis in higher plants. The occurrence, characterization and some regulatory properties of dihydrodipicolinate synthase.高等植物中赖氨酸生物合成的酶学。二氢二吡啶二羧酸合酶的存在、特性及一些调控特性。
FEBS Lett. 1977 Dec 15;84(2):236-40. doi: 10.1016/0014-5793(77)80696-0.
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