Zimmermann M H, Tomlinson P B
Science. 1966 Apr 1;152(3718):72-3. doi: 10.1126/science.152.3718.72.
The "optical shuttle" is an improved method of photographing with a motion-picture camera, one by one, sequential sections mounted on separate slides. The optical output from two microscopes is combined so that the images can be focused on a single film plane. Slides are photographed alternately through the microscopes. Simultaneous viewing of two successive sections in the microscopes enables initial precise alignment of images. Complex anatomical structures can thus be analyzed with relative ease.
“光学穿梭”是一种改进的用电影摄影机逐张拍摄安装在单独载玻片上的连续切片的方法。来自两台显微镜的光学输出被合并，以便图像能够聚焦在单个胶片平面上。通过显微镜交替拍摄载玻片。在显微镜中同时观察两个连续的切片能够对图像进行初始精确对齐。因此，可以相对轻松地分析复杂的解剖结构。
