日本根瘤菌的吸附对大豆细胞生长的抑制作用
Inhibition of Soybean Cell Growth by the Adsorption of Rhizobium japonicum.
作者信息
Ozawa T, Yamaguchi M
机构信息
Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai, Osaka, Japan.
出版信息
Plant Physiol. 1979 Jul;64(1):65-8. doi: 10.1104/pp.64.1.65.
Soybean cells in suspension culture were inhibited in their growth by mixed culture with Rhizobium japonicum 5033. Rhizobium cells had the ability to adsorb on the surface of soybean cells. Cell envelope prepared from Rhizobium by sonic oscillation inhibited the growth of soybean cells. The growth-inhibiting activity of the cell envelope was depressed by beta-glucosidase, KIO(4), urea, sodium cholate, and Triton X-100, but was stable on heating at 120 C for 15 minutes. Adsorption of the cell envelope on soybean cells was depressed by only beta-glucosidase. The sodium cholate-soluble fraction of the cell envelope had the growth-inhibiting activity. Results in this paper suggest that components of the Rhizobium cell surface cause the inhibition of soybean cell growth after the adsorption of the Rhizobium cell to the soybean cell.
悬浮培养的大豆细胞与日本根瘤菌5033混合培养时其生长受到抑制。根瘤菌细胞有吸附在大豆细胞表面的能力。通过超声振荡从根瘤菌制备的细胞包膜抑制大豆细胞的生长。细胞包膜的生长抑制活性被β-葡萄糖苷酶、高碘酸钾（KIO₄）、尿素、胆酸钠和曲拉通X-100降低，但在120℃加热15分钟时稳定。仅β-葡萄糖苷酶可降低细胞包膜在大豆细胞上的吸附。细胞包膜的胆酸钠可溶部分具有生长抑制活性。本文结果表明，根瘤菌细胞吸附到大豆细胞后，根瘤菌细胞表面成分导致大豆细胞生长受到抑制。
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