Matrai Adam Attila, Varga Adam, Fazekas Laszlo Adam, Bedocs-Barath Barbara, Nellamkuzhi Noel Johny, Nghi Tran Bao, Nemeth Norbert, Deak Adam
Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Moricz Zsigmond u. 22, H-4032 Debrecen, Hungary.
Metabolites. 2024 Apr 7;14(4):211. doi: 10.3390/metabo14040211.
As a rare complication of liver injury and certain interventions, bile can enter the bloodstream depending on the pressure gradient, resulting in bilhemia. Its micro-rheological and hemodynamic effects are still unclear. We aimed to study these parameters in experimental bilhemia models. Under general anesthesia, via laparotomy, bile was obtained by gallbladder puncture from pigs and by choledochal duct cannulation from rats. In vitro, 1 µL and 5 µL of bile were mixed with 500 µL of anticoagulated autologous blood. The systemic effect was also assessed (i.v. bile, 200 µL/bwkg). Hemodynamic and hematological parameters were monitored, and red blood cell (RBC) deformability and aggregation were determined. RBC deformability significantly decreased with the increasing bile concentration in vitro (1 µL: = 0.033; 5 µL: < 0.001) in both species. The RBC aggregation index values were concomitantly worsened (1 µL: < 0.001; 5 µL: < 0.001). The mean arterial pressure and heart rate decreased by 15.2 ± 6.9% and 4.6 ± 2.1% in rats (in 10.6 ± 2.6 s) and by 32.1 ± 14% and 25.2 ± 11.63% in pigs (in 48.3 ± 18.9 s). Restoration of the values was observed in 45 ± 9.5 s (rats) and 130 ± 20 s (pigs). Bilhemia directly affected the hemodynamic parameters and caused micro-rheological deterioration. The magnitude and dynamics of the changes were different for the two species.
作为肝损伤和某些干预措施的一种罕见并发症，胆汁可根据压力梯度进入血液循环，导致胆血症。其微观流变学和血流动力学效应仍不清楚。我们旨在研究实验性胆血症模型中的这些参数。在全身麻醉下，通过剖腹术，从猪的胆囊穿刺获取胆汁，从大鼠的胆总管插管获取胆汁。在体外，将1微升和5微升胆汁与500微升抗凝自体血混合。还评估了全身效应（静脉注射胆汁，200微升/体重千克）。监测血流动力学和血液学参数，并测定红细胞（RBC）的变形性和聚集性。在两个物种中，体外随着胆汁浓度的增加，红细胞变形性均显著降低（1微升：P = 0.033；5微升：P < 0.001）。红细胞聚集指数值也随之恶化（1微升：P < 0.001；5微升：P < 0.001）。大鼠的平均动脉压和心率在10.6±2.6秒内分别下降了15.2±6.9%和4.6±2.1%，猪的平均动脉压和心率在48.3±18.9秒内分别下降了32.1±14%和25.2±11.63%。在45±9.5秒（大鼠）和130±20秒（猪）观察到这些值恢复。胆血症直接影响血流动力学参数并导致微观流变学恶化。两个物种的变化幅度和动态不同。
