Department of Chemistry, University of Colorado, Boulder, Colorado 80309.
Plant Physiol. 1981 Mar;67(3):389-400. doi: 10.1104/pp.67.3.389.
The sequence of the glycosyl residues and the anomeric configurations of the glycosyl linkages of the acidic polysaccharides secreted by Rhizobium leguminosarum 128c53, Rhizobium leguminosarum 128c63, Rhizobium trifolii NA30, and Rhizobium trifolii 0403 have been determined. All four polysaccharides were found to have the following glycosyl repeating-unit structure, where galactosyl is Gal, glucosyl is Glc, glucuronosyl is GlcA, and pyruvyl is Pyr: [Formula: see text] Each of the glycosyl residues of these polysaccharides was determined to be in the d configuration and in the pyranose ring form. These results add support to the proposal that R. leguminosarum and R. trifolii have a particularly close genetic relationship. The significance of these results with regard to the possible function of these polysaccharides in the nodulation process is discussed.
根瘤菌属 128c53、根瘤菌属 128c63、三叶草根瘤菌 NA30 和三叶草根瘤菌 0403 分泌的酸性多糖的糖基残基序列和糖苷键的端基构型已被确定。这四种多糖都具有以下糖基重复单元结构,其中半乳糖基为 Gal,葡萄糖基为 Glc,葡萄糖醛酸基为 GlcA,丙酮酸基为 Pyr:[化学式:见正文]这些多糖的每个糖基残基都被确定为 d 构型和吡喃糖环形式。这些结果支持了根瘤菌属和三叶草根瘤菌具有特别密切的遗传关系的观点。讨论了这些结果对这些多糖在结瘤过程中可能的功能的意义。