Department of Genetics, University of Leeds, Leeds, LS2 9JT United Kingdom.
Plant Physiol. 1981 Sep;68(3):735-8. doi: 10.1104/pp.68.3.735.
Gametophore-over-producing mutants of the moss, Physcomitrella patens, when grown in liquid culture export high levels of cytokinin into their culture medium. The cytokinin produced by these mutants is postulated to account for their peculiar phenotype, that of mosses treated with exogenous cytokinin. N(6)-(Delta(2)-isopentenyl)adenine, the major cytokinin, has been identified previously in two of these mutants (Wang, Cove, Beutelmann, Hartmann 1980 Phytochemistry 19: 1103-1105) and now in additional representatives. A second cytokinin, zeatin, has been identified by its chromatographic behavior and mass spectrum including chemical ionization mass spectrometry of its permethyl derivative.
原丝体过量产生突变体的苔藓,拟南芥,当在液体培养中生长时,会将高水平的细胞分裂素分泌到培养基中。这些突变体产生的细胞分裂素被认为是造成它们特殊表型的原因,类似于用外源细胞分裂素处理的苔藓。N(6)-(Delta(2)-异戊烯基)腺嘌呤,主要的细胞分裂素,以前已经在其中两个突变体中被鉴定出来(Wang,Cove,Beutelmann,Hartmann 1980 Phytochemistry 19: 1103-1105),现在在更多的代表中也被鉴定出来。第二种细胞分裂素,玉米素,通过其色谱行为和质谱,包括其全甲基衍生物的化学电离质谱来鉴定。