北极光萼苔（叶苔纲）中内源吲哚-3-乙酸的测定
Determination of Endogenous Indole-3-Acetic Acid in Plagiochila arctica (Hepaticae).
作者信息
Law D M, Basile D V, Basile M R
机构信息
Department of Biology, The Pennsylvania State University, University Park, Pennsylvania 16802.
出版信息
Plant Physiol. 1985 Apr;77(4):926-9. doi: 10.1104/pp.77.4.926.
Abstract
Endogenous indole-3-acetic acid (IAA) was found in axenically cultured gametophytes of the leafy liverwort, Plagiochila arctica Bryhn and Kaal., by high-performance liquid chromatography with electrochemical detection. Identification of the methylated auxin was confirmed by gas chromatography-mass spectrometry. Addition of 57 micromolar IAA to cultures increased relative production of ethylene. This is the first definitive (gas chromatography-mass spectrometry) demonstration of the natural occurrence of IAA in a bryophyte.
摘要
通过高效液相色谱-电化学检测法，在无菌培养的北极叶苔（Plagiochila arctica Bryhn and Kaal.）配子体中发现了内源性吲哚-3-乙酸（IAA）。通过气相色谱-质谱法确认了甲基化生长素的鉴定。向培养物中添加57微摩尔IAA可增加乙烯的相对产量。这是首次在苔藓植物中明确（气相色谱-质谱法）证明IAA的天然存在。
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