Pflanzenphysiogisches Institut, University of Göttingen, Göttingen, Federal Republic of Germany.
Plant Physiol. 1982 Jul;70(1):113-6. doi: 10.1104/pp.70.1.113.
The effects of thioredoxin, dithioerythrol, and mixtures of both on enzymes involved in N metabolism of Chlorella sorokiniana have been studied. Glutamine synthetase, inactivated in vivo, was activated 8-fold by thioredoxin and dithioerythrol. By the same treatment, the activity of glutamate synthase was stimulated nearly 4-fold. Thus, two key enzymes of N metabolism were shown to be regulated via thioredoxin. The enzymes of the nitrate reducing system, i.e. nitrate reductase and nitrite reductase, were not affected by thiols. From these results, a model of NO(3) (-) metabolism is put forward which considers the regulating effect of light.
已研究了巯基化合物（如硫氧还蛋白、二硫苏糖醇）及其混合物对小球藻氮代谢相关酶的影响。体内失活的谷氨酰胺合成酶经巯基化合物和二硫苏糖醇处理后被激活 8 倍，谷氨酸合酶的活性也被刺激近 4 倍。因此，氮代谢的两个关键酶通过巯基化合物得到调节。硝酸盐还原系统的酶，如硝酸还原酶和亚硝酸还原酶，不受巯基化合物的影响。根据这些结果，提出了一个考虑光照调节作用的硝酸盐（NO3-）代谢模型。
