Use of microalgal-fungal pellets for hydroponics effluent recycling and high-value biomass production.

Hydroponic effluent (HE), enriched with inorganic nutrients, presents a viable, low-cost cultivation medium for microalgal biomass production and subsequent resource recovery. However, downstream processing, particularly biomass harvesting, remains a critical challenge for microalgal biorefineries. Therefore, the present study explored the potential of microalgal-fungal pellets (MAFP) in HE recycling for the production of biochemical-rich biomass. The optimized fungi-to-microalgae ratio (F:A) of 1:3 resulted in 100 % microalgal pelletization within 6 h. Surface characteristics suggested that metabolically active fungi with opposite charges facilitate microalgal pelletization. Further, MAFP exhibited a packed porous structure that was resilient to shear forces and had a high capacity for nutrient uptake. MAFP cultivation in HE demonstrated complete removal of ammonia-nitrogen (NH₃-N), phosphate (PO₄³⁻), and nitrate-nitrogen (NO₃⁻-N) within 7-9 days. The produced biomass was rich in biomolecules, including lipids (18.36 ± 0.12 % TS), protein (52.06 ± 2.1 % TS), and carbohydrates (28.95 ± 0.05 % TS). Besides, the high methane potential of MAFP (SMP ≈ 502.74 ± 19.1 mL CH g VS, and TMP ≈ 817.68 ± 12.5 mL CH g VS) indicated its suitability for biogas production. In essence, MAFP offers efficient HE recycling and biochemically rich biomass production, advancing towards a green and circular bioeconomy.