Laboratoire de Biochimie Vegetale, Institut de Botanique, 67083-Strasbourg Cedex, France.
Plant Physiol. 1983 Apr;71(4):756-62. doi: 10.1104/pp.71.4.756.
The roots of maize (Zea mays L. var LG11) seedlings were watered with a solution of Tridemorph (2,6-dimethyl-N-tridecyl-morpholine), a systemic fungicide, for 3 to 4 weeks from the onset of germination. Very few big up tri, open(5)-sterols, the major sterols of the control, were detected in the treated plants, and 9beta,19-cyclopropyl sterols accumulated dramatically in both roots and leaves. big up tri, open(8)-sterols were also found when low concentrations of the drug were used. The time course of the accumulation of the new sterols has been studied in plants treated with various concentrations (1-20 milligrams per liter) of Tridemorph. We found that: (a) cycloeucalenol-obtusifoliol isomerase, an enzyme opening the cyclopropane ring of cyclopropyl sterols, was strongly inhibited by the drug; and (b) the drug diffused readily from the roots to the whole plant and reached its enzymic targets in most of the leaf cells. The data obtained offer an opportunity to evaluate the physiological and biochemical consequence of the almost complete replacement of big up tri, open(5)-sterols by cyclopropyl sterols in higher plant cells.
从发芽开始，将玉米（Zea mays L. var LG11）幼苗的根部用一种三乙膦酸（2,6-二甲基-N-十三烷基吗啉）溶液处理 3 到 4 周。在处理过的植物中，很少检测到控制植物的主要甾醇大 up tri, open(5)-甾醇，而 9beta,19-环丙基甾醇在根和叶中都显著积累。当使用低浓度药物时，也发现了大 up tri, open(8)-甾醇。我们研究了用各种浓度（1-20 毫克/升）三乙膦酸处理的植物中新甾醇的积累时间过程。我们发现：（a）环羊毛甾醇-钝叶醇异构酶，一种打开环丙基甾醇中环丙烷环的酶，被药物强烈抑制；（b）药物从根部很容易扩散到整株植物，并在大多数叶片细胞中达到其酶靶标。这些数据为评估在高等植物细胞中，大 up tri, open(5)-甾醇几乎完全被环丙基甾醇取代的生理生化后果提供了机会。
