Raederstorff D, Rohmer M
Eur J Biochem. 1987 Apr 15;164(2):421-6. doi: 10.1111/j.1432-1033.1987.tb11074.x.
Tridemorph and fenpropimorph, two systemic fungicides known by their inhibitory effects on sterol biosynthesis in fungi and plants, were administered in vivo to the amoeba Acanthamoeba polyphaga. The compounds did not kill the cells, but modified completely their sterol pattern. Fungicide-exposed cells accumulated cyclopropylsterols indicating a partial blockage of the cyclopropane isomerase as in higher plants and delta 8-sterols indicating an inhibition of the delta 8----delta 7 isomerase as in fungi. Three new sterols, 4 alpha-methylergosta-9(11),24(28)-dienol, ergosta-6,8,22-trienol and poriferasta-6,8,22-trienol were isolated and identified, the former from control cells, the two latter from fungicide-treated cells. These results are in accordance with our previous results on the presence of cycloartenol as sterol precursor and confirm our hypothesis on a phylogenetic relationship of Acanthamoeba polyphaga with photosynthetic phyla.
十三吗啉和粉唑醇是两种内吸性杀菌剂，因其对真菌和植物中甾醇生物合成的抑制作用而闻名。它们被用于对多食棘阿米巴进行体内给药。这些化合物并未杀死细胞，但完全改变了它们的甾醇模式。接触杀菌剂的细胞积累了环丙基甾醇，这表明环丙烷异构酶部分受阻，就像在高等植物中一样；同时还积累了δ8-甾醇，这表明δ8→δ7异构酶受到抑制，就像在真菌中一样。三种新的甾醇，即4α-甲基麦角甾-9(11),24(28)-二烯醇、麦角甾-6,8,22-三烯醇和海绵甾-6,8,22-三烯醇被分离并鉴定出来，前者来自对照细胞，后两者来自经杀菌剂处理的细胞。这些结果与我们之前关于环阿屯醇作为甾醇前体存在的结果一致，并证实了我们关于多食棘阿米巴与光合门之间系统发育关系的假设。
