与菠菜叶绿体包膜相关的类胡萝卜素合酶和类胡萝卜素脱氢酶。

Phytoene synthase and phytoene dehydrogenase associated with envelope membranes from spinach chloroplasts.

机构信息

Institut für Biologie II, Zellbiologie, Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.

出版信息

Planta. 1982 Nov;156(2):176-80. doi: 10.1007/BF00395433.

DOI:10.1007/BF00395433

PMID:24272314

Abstract

Envelope membranes of spinach chloroplasts contain appreciable activities of the carotenogenic enzymes phytoene synthase (formation of phytoene by condensation of two molecules geranylgeranyl pyrophosphate) and phytoene dehydrogenase (formation of lycopene from phytoene), plus a phosphatase activity. These results were obtained by coincubation experiments using isolated envelope membranes and either a phytoene-forming in vitro system (from [1-(14)C]isopentenyl pyrophosphate) or [(14)C]geranylgeranyl pyrophosphate or a geranylgeranyl-pyrophosphate-forming in vitro system (from [1-(14)C]isopentenyl pyrophosphate). Within thylakoids carotenogenic enzymes could not be detected. It is concluded that the chloroplast envelope is at least a principal site of the membrane-bound steps of carotenoid biosynthesis in chloroplasts.

摘要

菠菜叶绿体的包膜含有相当数量的类胡萝卜素生物合成酶的活性，如八氢番茄红素合酶（由两个焦磷酸香叶基香叶基转移酶分子缩合形成八氢番茄红素）和八氢番茄红素脱氢酶（由八氢番茄红素形成番茄红素），以及磷酸酶活性。这些结果是通过使用分离的包膜与类胡萝卜素形成的体外系统（来自[1-(14)C]异戊烯焦磷酸）或[(14)C]香叶基香叶基焦磷酸或香叶基香叶基焦磷酸形成的体外系统（来自[1-(14)C]异戊烯焦磷酸）进行共孵育实验获得的。在类囊体中未检测到类胡萝卜素生物合成酶。因此，可以得出结论，叶绿体包膜至少是叶绿体中类胡萝卜素生物合成膜结合步骤的主要部位。