Block M A, Joyard J, Douce R
Biochim Biophys Acta. 1980 Aug 1;631(1):210-9. doi: 10.1016/0304-4165(80)90069-0.
Chloroplasts isolated from fully developed spinach leaves and incubated in the presence of isopentenyl pyrophosphate were able to synthesize rapidly geranylgeranyl chlorophyll alpha and geranylgeraniol. The biosynthesis of the geranylgeraniol derivatives from isopentenyl pyrophosphate is a compartimentalized process. The membrane fractions (thylakoid and envelope membranes) were essentially unable to synthesize geranylgeraniol, geranylgeranyl pyrophosphate and geranylgeranyl chlorophyll alpha. When stromal and thylakoid fractions were combined the capacity to synthesize geranylgeranyl chlorophyll alpha and geranylgeraniol was restored. When stromal and envelope membrane fractions were combined the capacity to synthesize gernylgeranyl pyrophosphate and geranylgeraniol was restored. The products of the reaction were discharged inside the lipid phase of the membranes.
从完全成熟的菠菜叶中分离出的叶绿体，在异戊烯基焦磷酸存在的情况下进行孵育，能够快速合成香叶基香叶基叶绿素α和香叶基香叶醇。从异戊烯基焦磷酸合成香叶基香叶醇衍生物的生物合成是一个区室化过程。膜部分（类囊体膜和包膜膜）基本上无法合成香叶基香叶醇、香叶基香叶基焦磷酸和香叶基香叶基叶绿素α。当基质部分和类囊体部分混合时，合成香叶基香叶基叶绿素α和香叶基香叶醇的能力得以恢复。当基质部分和包膜膜部分混合时，合成香叶基香叶基焦磷酸和香叶基香叶醇的能力得以恢复。反应产物被排放到膜的脂质相内部。
