Michigan State University, MSU-DOE Plant Research Laboratory, East Lansing, Michigan 48824.
Plant Physiol. 1983 May;72(1):86-9. doi: 10.1104/pp.72.1.86.
Application of gibberellin A(53) (GA(53)) to short-day (SD)-grown spinach (Spinacia oleracea L.) plants caused an increase in petiole length and leaf angle similar to that found in plants transferred to long days (LD). [(2)H] GA(53) was fed to plants in SD, LD, and in a SD to LD transition experiment, and the metabolites were identified by gas chromatography with selected ion monitoring. After 2, 4, or 6 SD, [(2)H]GA(53) was converted to [(2)H]GA(19) and [(2)H]GA(44). No other metabolites were detected. After 2 LD, only [(2)H] GA(20) was identified. In the transition experiment in which plants were given 4 SD followed by 2 LD, all three metabolites were found. The results demonstrate unequivocally that GA(19), GA(20), and GA(44) are metabolic products of GA(53), and strongly suggest that photoperiod regulates GA metabolism, in part, by controlling the conversion of GA(19) to GA(20).
赤霉素 A(53)（GA(53)）应用于短日照（SD）生长的菠菜（Spinacia oleracea L.）植株中，可使叶柄长度和叶片角度增加，类似于转移到长日照（LD）的植株。[(2)H]GA(53)被喂给 SD、LD 和 SD 到 LD 转换实验中的植物，通过气相色谱法和选择离子监测法鉴定代谢物。在 2、4 或 6 SD 后，[(2)H]GA(53)被转化为[(2)H]GA(19)和[(2)H]GA(44)。未检测到其他代谢物。在 2 LD 后，仅鉴定出[(2)H]GA(20)。在以 4 SD 随后是 2 LD 给予植物的过渡实验中，发现了这三种代谢物。结果明确表明 GA(19)、GA(20)和 GA(44)是 GA(53)的代谢产物，并强烈表明光周期通过控制 GA(19)向 GA(20)的转化来部分调节 GA 代谢。
