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本文引用的文献

1
Gibberellin metabolism in cell-free extracts from spinach leaves in relation to photoperiod.叶绿体游离体系中赤霉素代谢与光周期的关系
Plant Physiol. 1986 Sep;82(1):190-5. doi: 10.1104/pp.82.1.190.
2
Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures.莨菪碱6β-羟化酶，一种依赖2-氧代戊二酸的双加氧酶，存在于产生物碱的根培养物中。
Plant Physiol. 1986 Jun;81(2):619-25. doi: 10.1104/pp.81.2.619.
3
Effect of photoperiod on the metabolism of deuterium-labeled gibberellin a(53) in spinach.光照周期对菠菜中氘标记赤霉素 A(53)代谢的影响。
Plant Physiol. 1983 May;72(1):86-9. doi: 10.1104/pp.72.1.86.
4
Photoperiodic control of gibberellin metabolism in spinach.菠菜中赤霉素代谢的光周期调控
Plant Physiol. 1982 Feb;69(2):287-91. doi: 10.1104/pp.69.2.287.
5
Effect of Photoperiod on the Levels of Endogenous Gibberellins in Spinach as Measured by Combined Gas Chromatography-selected Ion Current Monitoring.光照周期对菠菜内源赤霉素水平的影响，采用气相色谱-选择离子流监测法测定。
Plant Physiol. 1980 Nov;66(5):844-6. doi: 10.1104/pp.66.5.844.
6
Effects of photoperiod on growth rate and endogenous gibberellins in the long-day rosette plant spinach.光周期对长日照莲座状植物菠菜生长速率和内源赤霉素的影响。
Plant Physiol. 1971 Jun;47(6):821-7. doi: 10.1104/pp.47.6.821.
7
Transition state analogs as ligands for affinity purification of juvenile hormone esterase.过渡态类似物作为用于亲和纯化保幼激素酯酶的配体。
Science. 1986 Sep 5;233(4768):1073-6. doi: 10.1126/science.3738525.
8
Elicitor-induced prolyl hydroxylase from French bean (Phaseolus vulgaris). Localization, purification and properties.菜豆（菜豆属）中诱导子诱导的脯氨酰羟化酶。定位、纯化及特性
Biochem J. 1985 Aug 1;229(3):693-9. doi: 10.1042/bj2290693.
9
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.

从菠菜叶片中部分纯化赤霉素氧化酶。

Partial purification of gibberellin oxidases from spinach leaves.

机构信息

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.

出版信息

Plant Physiol. 1987 Sep;85(1):87-90. doi: 10.1104/pp.85.1.87.

DOI:10.1104/pp.85.1.87
PMID:16665690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1054208/
Abstract

Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA(12) --> GA(53) --> GA(44) --> GA(19) --> GA(20). Two of these, GA(53) oxidase and GA(19) oxidase, were separable from the other two, GA(44) oxidase and GA(12) 13-hydroxylase, by anion exchange high performance liquid chromatography (HPLC). Apparent molecular weights of the four enzymes as determined by gel filtration HPLC are: GA(12) 13-hydroxylase, 28,400; GA(53) oxidase, 42,500; GA(44) oxidase, 38,100; GA(19) oxidase, 39,500. GA(44) oxidase was purified approximately 100-fold in 0.3% yield by a combination of ammonium sulfate fractionation, anion exchange HPLC, phenyl-Sepharose chromatography and gel filtration HPLC.

摘要

四种酶活性催化了赤霉素（GA）生物合成途径中的以下氧化步骤，这些酶活性在菠菜（Spinacia oleracea L.）叶片暴露于 8 个长日之后被提取出来：GA(12) --> GA(53) --> GA(44) --> GA(19) --> GA(20)。其中两种，GA(53)氧化酶和 GA(19)氧化酶，可以通过阴离子交换高效液相色谱（HPLC）从另外两种酶，GA(44)氧化酶和 GA(12) 13-羟化酶中分离出来。通过凝胶过滤 HPLC 测定，这四种酶的表观分子量分别为：GA(12) 13-羟化酶，28400；GA(53)氧化酶，42500；GA(44)氧化酶，38100；GA(19)氧化酶，39500。GA(44)氧化酶通过硫酸铵分级沉淀、阴离子交换 HPLC、苯基-Sepharose 色谱和凝胶过滤 HPLC 的组合，以 0.3%的产率被大约纯化了 100 倍。