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β3-肾上腺素能受体5'侧翼区域中的多个潜在调控元件。

Multiple potential regulatory elements in the 5' flanking region of the beta 3-adrenergic receptor.

作者信息

Liggett S B, Schwinn D A

机构信息

Department of Medicine (Pulmonary), Duke University School of Medicine, Durham, NC 27710.

出版信息

DNA Seq. 1991;2(1):61-3. doi: 10.3109/10425179109008441.

DOI:10.3109/10425179109008441
PMID:1666313
Abstract

Since the promoter regions of several genes which modulate intracellular cAMP contain cAMP responsive elements which participate in the regulation of transcription, we assessed whether similar elements were present in the 5' flanking region of the recently cloned human beta 3-adrenergic receptor. This receptor is coupled to cAMP formation, and may play a key role in adrenergic receptor-mediated lipolysis and thermogenesis. Therefore, from a human genomic library we cloned the beta 3-AR coding block and a 1,320 base pair region immediately 5' upstream from the initiation of translation. Based on the classic consensus sequence, we found four potential cAMP responsive elements in this 5' flanking region: CGAGGTCA, TCACATCA, TGAGGTCT and TGACTCCA.

摘要

由于几个调节细胞内cAMP的基因的启动子区域包含参与转录调节的cAMP反应元件，我们评估了在最近克隆的人β3 - 肾上腺素能受体的5'侧翼区域是否存在类似元件。该受体与cAMP形成偶联，并可能在肾上腺素能受体介导的脂解和产热中起关键作用。因此，我们从人基因组文库中克隆了β3 - AR编码区以及翻译起始点上游5'端紧邻的1320个碱基对区域。基于经典的共有序列，我们在该5'侧翼区域发现了四个潜在的cAMP反应元件：CGAGGTCA、TCACATCA、TGAGGTCT和TGACTCCA。

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Interaction of nuclear factors with the cAMP response elements of the human β(3)-adrenoceptor gene.核因子与人β(3) -肾上腺素能受体基因的环磷酸腺苷反应元件的相互作用。
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New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
Nucleic Acids Res. 1992 Feb 25;20(4):935-58. doi: 10.1093/nar/20.4.935.
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