Searles R P, Nipper V J, Machida C A
Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton 97006.
DNA Seq. 1994;4(4):231-41. doi: 10.3109/10425179409020846.
We have cloned the gene for the rhesus macaque beta 1-adrenergic receptor. In addition to the protein coding block, we have sequenced its 5' (1424 bp) and 3' (1534 bp) flanking regions and aligned them with comparable sections of the rat beta 1-adrenergic receptor gene. The rhesus macaque gene contains a 1440 bp open reading frame which codes for a deduced protein of 480 amino acids that is 95% and 89% similar to the human and rat beta 1-adrenergic receptors, respectively. The rhesus macaque beta 1-adrenergic receptor contains conserved sites for potential N-linked glycosylation and cAMP-dependent protein kinase phosphorylation identified within the human and rat receptors, but differs in the structure and length of the third cytoplasmic loop. The 400 bases of 5' flanking sequence proximal to the protein coding block are highly conserved (84% similarity) between the rat and rhesus macaque genes. The entire 3' flanking sequence, which extends beyond two potential polyadenylation sites at 1050 and 1337 bp relative to the translation termination codon, is also highly conserved between the two species. Comparison of the flanking sequences of the two species reveals conserved regulatory sequences which may be important for beta 1-adrenergic receptor expression and transcriptional modulation.
我们已经克隆了恒河猴β1 - 肾上腺素能受体的基因。除了蛋白质编码区外，我们还对其5'端（1424 bp）和3'端（1534 bp）侧翼区域进行了测序，并将它们与大鼠β1 - 肾上腺素能受体基因的相应区域进行了比对。恒河猴基因包含一个1440 bp的开放阅读框，编码一个推导的480个氨基酸的蛋白质，该蛋白质与人类和大鼠的β1 - 肾上腺素能受体分别有95%和89%的相似性。恒河猴β1 - 肾上腺素能受体含有在人类和大鼠受体中鉴定出的潜在N - 糖基化和cAMP依赖性蛋白激酶磷酸化的保守位点，但在第三个细胞质环的结构和长度上有所不同。蛋白质编码区近端的5'侧翼序列的400个碱基在大鼠和恒河猴基因之间高度保守（84%相似性）。相对于翻译终止密码子，延伸超过两个潜在聚腺苷酸化位点（分别位于1050和1337 bp处）的整个3'侧翼序列在这两个物种之间也高度保守。对这两个物种侧翼序列的比较揭示了保守的调控序列，这些序列可能对β1 - 肾上腺素能受体的表达和转录调控很重要。
