Lehrstuhl für Biochemie der Pflanze, Universität Göttingen, Untere Karspüle 2, 3400 Göttingen, Federal Republic of Germany.
Plant Physiol. 1983 Aug;72(4):1139-41. doi: 10.1104/pp.72.4.1139.
The subcellular distribution of fructose 2,6-bisphosphate in spinach (Spinacia oleracea) leaves was studied using nonaqueous fractionation, showing that all, or almost all, is located in the cytosol. The amount of fructose 2,6-bisphosphate present in leaves during the diurnal cycle was measured and compared to the accumulation of starch and sucrose, and the amounts of selected phosphorylated intermediates in the leaf. Upon illumination, the level of fructose 2,6-bisphosphate decreases, but prolonged illumination leads to an increase in the level to above that found in the dark, which accompanies the onset of rapid accumulation of starch in the leaf.
采用非水相分级分离法研究了菠菜（Spinacia oleracea）叶片中果糖 2,6-二磷酸的亚细胞分布，表明其全部或几乎全部位于细胞质中。测量了叶片在昼夜周期中存在的果糖 2,6-二磷酸的量，并与淀粉和蔗糖的积累以及叶片中选定的磷酸化中间产物的量进行了比较。在光照下，果糖 2,6-二磷酸的水平降低，但长时间的光照会导致其水平升高到高于黑暗中的水平，这伴随着淀粉在叶片中快速积累的开始。
