Stitt M, Cseke C, Buchanan B B
Eur J Biochem. 1984 Aug 15;143(1):89-93. doi: 10.1111/j.1432-1033.1984.tb08345.x.
Fructose-6-phosphate 2-kinase and fructose-2,6-bisphosphatase have been partially purified from spinach leaves and their regulatory properties studied. Fructose-6-phosphate 2-kinase was activated by phosphate and fructose 6-phosphate, and inhibited by 3-phosphoglycerate and dihydroxyacetone phosphate. Fructose-2,6-bisphosphatase was inhibited by fructose 6-phosphate and phosphate. The interaction between these effectors was studied when they were varied, alone or in combination, over a range of concentrations representative of those in the cytosol of spinach leaf cells. In conditions when dihydroxyacetone phosphate or 3-phosphoglycerate rise, as is typical during photosynthesis, the fructose 2,6-bisphosphate level will decrease, which will favour sucrose synthesis. In conditions when fructose 6-phosphate accumulates, fructose 2,6-bisphosphate should rise, which will favour a restriction of sucrose synthesis and promotion of starch synthesis.
已从菠菜叶中部分纯化出6-磷酸果糖-2-激酶和果糖-2,6-二磷酸酶，并对其调节特性进行了研究。6-磷酸果糖-2-激酶被磷酸和6-磷酸果糖激活，并被3-磷酸甘油酸和磷酸二羟丙酮抑制。果糖-2,6-二磷酸酶被6-磷酸果糖和磷酸抑制。当这些效应物单独或组合在代表菠菜叶细胞胞质溶胶中浓度的一系列浓度范围内变化时，研究了它们之间的相互作用。在光合作用期间典型的磷酸二羟丙酮或3-磷酸甘油酸升高的情况下，果糖-2,6-二磷酸水平将降低，这将有利于蔗糖合成。在6-磷酸果糖积累的情况下，果糖-2,6-二磷酸应该升高，这将有利于限制蔗糖合成并促进淀粉合成。
