United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705.
Plant Physiol. 1983 Sep;73(1):130-4. doi: 10.1104/pp.73.1.130.
High resolution growth recording techniques and reverse isotope dilution analysis were used to study the relationship between indole-3-acetic acid (IAA) concentration and curvature of excised bean (Phaseolus vulgaris L. cv Bush Burpee Stringless) first internode sections unilaterally treated with hormone. The maximum rate of curvature occurred rapidly (within 25 minutes) and was proportional to the log of the amount of applied IAA recovered in the tissue. The rate of curvature decreased after 30 minutes although little or no lateral migration of applied IAA occurred and tissue levels of IAA increased. The biologic activity of IAA-amino acid conjugates was found to be directly related to the amount of free IAA, resulting from their hydrolysis, which could be recovered from the tissue.
采用高分辨率生长记录技术和反向同位素稀释分析方法,研究了单侧用激素处理的离体菜豆(Phaseolus vulgaris L. cv Bush Burpee Stringless)第一节间吲哚乙酸(IAA)浓度与曲率之间的关系。最大曲率速率发生得很快(在 25 分钟内),并与组织中回收的施加 IAA 的量的对数成正比。尽管施加的 IAA 很少或没有横向迁移,并且组织中的 IAA 水平增加,但 30 分钟后曲率速率下降。发现 IAA-氨基酸缀合物的生物活性与从组织中回收的可水解的游离 IAA 的量直接相关。