U.S. Department of Agriculture, Agricultural Research Service-West, Beltsville, Maryland 20705.
Plant Physiol. 1989 Jun;90(2):398-400. doi: 10.1104/pp.90.2.398.
Gas chromatography-selected ion monitoring-mass spectral analysis using [(13)C(6)]indole-3-acetic acid (IAA) as an internal standard provides an effective means for quantitation of IAA liberated during direct strong basic hydrolysis of bean (Phaseolus vulgaris L.) seed powder, provided that extra precautions are undertaken to exclude oxygen from the reaction vial. Direct seed powder hydrolysis revealed that the major portion of amide IAA conjugates in bean seeds are not extractable by aqueous acetone, the solvent used commonly for IAA conjugate extraction from seeds and other plant tissues. Strong basic hydrolysis of plant tissue can be used to provide new information on IAA content.
采用 [(13)C(6)]吲哚-3-乙酸(IAA)作为内标,通过气相色谱选择离子监测-质谱分析,为直接强堿水解菜豆(Phaseolus vulgaris L.)种子粉末过程中释放的 IAA 进行定量分析提供了有效手段,但需要特别注意排除反应小瓶中的氧气。直接种子粉末水解表明,菜豆种子中酰胺 IAA 轭合物的大部分不能用常用的种子和其他植物组织中 IAA 轭合物提取的水-丙酮提取。植物组织的强堿水解可用于提供关于 IAA 含量的新信息。