Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan.
Plant Physiol. 1983 Sep;73(1):82-6. doi: 10.1104/pp.73.1.82.
Mitochondrial preparations isolated from black gram (Vigna mungo L.) cotyledons exhibited cyanide-resistant respiration which was of mitochondrial origin. The appearance and the disappearance of this alternative respiration took place during and following imbibition. During the first 6 hours of imbibition, the respiration was completely inhibited by cyanide, but after this time the alternative respiration markedly developed, reaching a maximal cyanide-resistance 12 to 16 hours after the start of imbibition. Subsequently, the alternative respiration gradually disappeared. The actions of cycloheximide and chloramphenicol indicated that the appearance was dependent on cytoplasmic protein synthesis and that the disappearance depended on both cytoplasmic and mitochondrial protein synthesis. The alternative pathway contributed to state 4 respiration, but not to state 3 respiration, in mitochondria from 1-day-old cotyledons. On day 3, it contributed to neither state 3 nor state 4.
从黑眼豆(Vigna mungo L.)子叶中分离得到的线粒体制剂表现出对氰化物具有抗性的呼吸作用,该呼吸作用源自线粒体。这种替代呼吸作用在吸胀过程中和吸胀之后出现和消失。在吸胀的头 6 个小时内,呼吸作用完全被氰化物抑制,但在此之后,替代呼吸作用显著发展,在吸胀开始 12 至 16 小时后达到最大的氰化物抗性。随后,替代呼吸作用逐渐消失。环己亚胺和氯霉素的作用表明,这种替代呼吸作用的出现依赖于细胞质蛋白合成,而消失则依赖于细胞质和线粒体蛋白合成。在来自 1 天大的子叶的线粒体中,替代途径有助于状态 4 的呼吸作用,但对状态 3 的呼吸作用没有影响。在第 3 天,它既不影响状态 3,也不影响状态 4。