Kerson G W, Miernyk J A, Budd K
Department of Biology, Queen's University, Kingston, Ontario K7L 3N6 Canada.
Plant Physiol. 1984 May;75(1):222-4. doi: 10.1104/pp.75.1.222.
Phosphate uptake by the blue-green alga Oscillatoria limnetica Lemmerman is stimulated by micromolar concentrations of Ca(2+). The calmodulin antagonists 4-(3-[2(trifluoromethyl)phenylthiazin-10-yl]propyl)-1-piperazine ethanol-HCl and its monofluoro-analog inhibit orthophosphate uptake of Oscillatoria limnetica by over 97% implying involvement of calmodulin in this process. A calmodulin-like protein was quantitated in cell-free extracts from O. limnetica by radioimmunoassay.
微摩尔浓度的Ca(2+)可刺激蓝绿藻湖沼颤藻（Oscillatoria limnetica Lemmerman）对磷酸盐的吸收。钙调蛋白拮抗剂4-(3-[2(三氟甲基)苯基噻嗪-10-基]丙基)-1-哌嗪乙醇盐酸盐及其单氟类似物可抑制湖沼颤藻对正磷酸盐的吸收达97%以上，这表明钙调蛋白参与了这一过程。通过放射免疫测定法定量了湖沼颤藻无细胞提取物中的一种类钙调蛋白。
