Kanabus J, Pikaard C S, Cherry J H
Horticulture Department, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1984 Jul;75(3):639-44. doi: 10.1104/pp.75.3.639.
Tobacco (Nicotiana tabacum L. cv Wisconsin 38) cells grown in suspension culture at 26 degrees C produce heat shock proteins (HSPs) when exposed to elevated temperature of 34 to 42 degrees C. At 34 and 38 degrees C, synthesis of normal proteins is maintained while HSPs are expressed within 30 minutes after initiation of the shock. At 42 degrees C, HSPs are still expressed but normal proteins are made at a reduced rate or not at all. Exposure of cells to 38 degrees C allows for a full expression of HSPs without inhibition of the synthesis of normal proteins. Induced synthesis of HSPs at 38 degrees C is maximal 1 to 2 hours after elevation of temperature and diminishes thereafter through at least 6 hours. Cells growing asynchronously in the logarithmic phase of growth produce HSPs at a much higher rate than those in the stationary phase. The ability to synthesize HSPs disappears about one generation time before the cells reach a growth plateau.
在26℃下进行悬浮培养的烟草（Nicotiana tabacum L. cv Wisconsin 38）细胞，当暴露于34至42℃的高温时会产生热休克蛋白（HSPs）。在34℃和38℃时，正常蛋白质的合成得以维持，而热休克蛋白在热激开始后30分钟内表达。在42℃时，热休克蛋白仍会表达，但正常蛋白质的合成速率降低或完全停止。将细胞暴露于38℃可使热休克蛋白充分表达，而不会抑制正常蛋白质的合成。在38℃时，热休克蛋白的诱导合成在温度升高后1至2小时达到最大值，此后至少6小时逐渐减少。在对数生长期异步生长的细胞产生热休克蛋白的速率比处于稳定期的细胞高得多。在细胞达到生长平台期前约一代时间，合成热休克蛋白的能力消失。
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