Mosser D D, Heikkila J J, Bols N C
J Cell Physiol. 1986 Sep;128(3):432-40. doi: 10.1002/jcp.1041280312.
Cultures of the rainbow trout fibroblast cell line RTG-2 withstood temperatures from 0 degrees C to 28 degrees C. At 0 degrees C and 28 degrees C, no proliferation occurred, but cells persisted for at least 7 days. If the cultures were placed back at 22 degrees C, proliferation returned to normal in those that had been kept at 0 degrees C but was reduced in cultures that had been kept at 28 degrees C. Above 28 degrees C, cultures survived for only short periods. If RTG-2 cells that were grown routinely at 22 degrees C were shifted to 26, 28, and 30 degrees C, heat shock proteins (hsps) of 100, 87, 70, 68, 60, 39, 27, and 19 kilodaltons were synthesized. Synthesis was most pronounced at 28 degrees C, and at this temperature hsp synthesis was maximal by 2 hr and had returned to control levels by 36 hr. Individual hsps were synthesized maximally at slightly different times and temperatures, but under all conditions hsps 87 and 70 were most abundant. If cultures were shifted to 24 degrees C or 32 degrees C, hsp synthesis was not observed. Neither the placement of cultures at 5 degrees C nor the shift of cultures that had been maintained at 0 degrees C or 5 degrees C back to 22 degrees C induced the synthesis of hsps. However, cultures incubated at 5 degrees C for 24 hr did synthesize hsps at 26 degrees C, 28 degrees C, and 30 degrees C.
虹鳟鱼成纤维细胞系RTG - 2的培养物能耐受0摄氏度至28摄氏度的温度。在0摄氏度和28摄氏度时，细胞不发生增殖，但能存活至少7天。如果将培养物放回22摄氏度，在0摄氏度保存过的培养物中细胞增殖恢复正常，但在28摄氏度保存过的培养物中增殖减少。高于28摄氏度时，培养物只能存活很短时间。如果将常规在22摄氏度培养的RTG - 2细胞转移至26、28和30摄氏度，会合成100、87、70、68、60、39、27和19千道尔顿的热休克蛋白（hsps）。在28摄氏度时合成最为明显，在此温度下hsp合成在2小时达到最大值，并在36小时恢复到对照水平。各个hsp在略有不同的时间和温度下合成量最大，但在所有条件下，hsp87和hsp70最为丰富。如果将培养物转移至24摄氏度或32摄氏度，则未观察到hsp合成。将培养物置于5摄氏度以及将在0摄氏度或5摄氏度保存过的培养物转移回22摄氏度均未诱导hsp合成。然而，在5摄氏度孵育24小时的培养物在26摄氏度、28摄氏度和30摄氏度时确实会合成hsp。
