Department of Horticultural Science and Landscape Architecture, University of Minnesota, St. Paul, Minnesota 55108.
Plant Physiol. 1984 Dec;76(4):955-8. doi: 10.1104/pp.76.4.955.
Concentrations of abscisic acid (ABA) and indole-3-acetic acid (IAA) in the second most recently expanded trifoliolate leaf were determined during reproductive development of soybean (Glycine max [L.] Merr cv ;Chippewa 64'). The concentration of ABA in leaves was constant during most of the seed filling period until the seeds began to dry. The concentration of IAA in the leaves decreased throughout development. Removal of pods 36 hours prior to sampling resulted in increased concentrations of ABA in leaves during the period of rapid pod filling but had little effect on the concentration of IAA in leaves. ABA appears to accumulate in leaves after fruit removal only when fruits represent the major sink for photosynthate.ABA and IAA moving acropetally and basipetally in petioles of soybean were estimated using a phloem exudation technique. ABA was found to move mostly in the basipetal direction in petioles (away from laminae). IAA, primarily in the form of ester conjugate(s), was found to be moving acropetally (toward laminae) in petioles. The highest amount of IAA ester(s) was found in petiole exudate during the mid and late stages of seed filling. Removal of fruits 36 hours prior to exudation reduced the amount of IAA ester recovered in exudate, suggesting that fruits were a source of the IAA conjugate in petiole exudate.
在大豆（Glycine max [L.] Merr cv；Chippewa 64'）生殖发育过程中，测定了第二对新近展开的复叶中叶片中脱落酸（ABA）和吲哚-3-乙酸（IAA）的浓度。叶片中 ABA 的浓度在种子填充期的大部分时间保持不变，直到种子开始干燥。叶片中 IAA 的浓度在整个发育过程中逐渐下降。在取样前 36 小时去除豆荚会导致快速荚果填充期间叶片中 ABA 浓度增加，但对叶片中 IAA 浓度几乎没有影响。只有当果实成为光合产物的主要汇时，ABA 似乎才会在去除果实后积累在叶片中。ABA 和 IAA 在大豆叶柄中的向顶和向基运动，采用韧皮部泌出技术进行了估计。发现 ABA 主要沿叶柄的向基方向（远离叶片）移动。IAA，主要以酯轭合物的形式，被发现沿叶柄向顶（向叶片）移动。在种子填充的中期和后期，叶柄泌出物中发现的 IAA 酯（s）最多。在泌出前 36 小时去除果实会减少泌出物中 IAA 酯的回收量，表明果实是叶柄泌出物中 IAA 轭合物的来源。
