采用新型高效液相色谱法测定植物组织中吲哚-3-乙酸的含量
Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method.
作者信息
Sweetser P B, Swartzfager D G
机构信息
E. I. du Pont de Nemours and Company, Central Research and Development Department, Experimental Station, Wilmington, Delaware 19898.
出版信息
Plant Physiol. 1978 Feb;61(2):254-8. doi: 10.1104/pp.61.2.254.
Abstract
A method for the analysis of indole-3-acetic acid (IAA) in plant extracts has been developed based on high performance liquid chromatography separation of IAA on a microparticulate strong anion exchange column followed by quantitation with two selective detectors: an electrochemical, carbon paste amperometric detector and/or a fluorescence detector. The detection limit for IAA is less than 1 nanogram with the fluorescence detector and less than 50 picograms with the electrochemical detector.The IAA levels are reported for various tissues of wheat, pinto beans, soybeans, cotton, and corn.
摘要
基于在微粒强阴离子交换柱上对吲哚 - 3 - 乙酸（IAA）进行高效液相色谱分离，随后使用两种选择性检测器进行定量分析，开发了一种用于分析植物提取物中IAA的方法：一种是电化学碳糊安培检测器，另一种是荧光检测器。使用荧光检测器时，IAA的检测限小于1纳克；使用电化学检测器时，检测限小于50皮克。报告了小麦、斑豆、大豆、棉花和玉米不同组织中的IAA水平。
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