Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California 90024.
Plant Physiol. 1985 Mar;77(3):753-7. doi: 10.1104/pp.77.3.753.
Ethylene treatment of carrot roots elicits a developmental program encompassing an increase in respiration rate and changes in gene expression. Both phenomena are potentiated when ethylene is administered in O(2). Our previous studies showed that both respiration and a number of ethylene specific mRNAs increase together in response to ethylene through some 21 hours, whereas thereafter respiration continues to rise, while the level of induced mRNAs drops. Herein we ask whether an experimentally effected drop in the respiration rate within the first 21 hours caused by the withdrawal of ethylene, or substitution of air for O(2) in the continued presence of ethylene, is linked to a drop in the level of ethylene-induced mRNA. Quantitative estimation of two ethylene evoked mRNAs by dot blot hybridization with appropriate cDNA clones has shown that under the specified treatment the induced mRNA levels remain constant while the respiration rate drops, suggesting that gene expression, as reflected in induced mRNA levels, and respiration rate are separately regulated facets of the ethylene response.
乙烯处理胡萝卜根会引发一个发育程序，包括呼吸速率的增加和基因表达的变化。当乙烯在氧气中施用时，这两种现象都会增强。我们之前的研究表明，在大约 21 小时内，乙烯通过某些途径刺激呼吸和许多乙烯特异性 mRNA 一起增加，而此后呼吸继续上升，而诱导的 mRNA 水平下降。在这里，我们想问的是，在最初的 21 小时内，由于乙烯的撤离或在继续存在乙烯的情况下用空气替代氧气，实验性地降低呼吸速率是否与乙烯诱导的 mRNA 水平的下降有关。通过用适当的 cDNA 克隆进行斑点杂交对两种乙烯诱发的 mRNA 进行定量估计表明，在指定的处理下，呼吸速率下降时，诱导的 mRNA 水平保持不变，这表明基因表达（如诱导的 mRNA 水平所反映的）和呼吸速率是乙烯反应的独立调节方面。
