Department of Chemistry and Biochemistry, University of California, 405 Hilgard Ave., 90024, Los Angeles, CA, U.S.A..
Plant Mol Biol. 1984 Nov;3(6):393-401. doi: 10.1007/BF00033387.
The plant hormone ethylene elicits many biochemical changes in target tissues. To investigate ethylene effects on expression of genetic information, cDNA clones corresponding to ethylene-induced carrot root mRNAs were constructed and isolated. RNA dot blot analysis showed that for the three clones studied peak cytosolic mRNA prevalence occurred at 21 h of treatment followed thereafter by rapid messenger decay. DNA filter excess hybridization to in vitro synthesized nuclear RNA showed that the ethylene-induced mRNA increase is engendered by transcription of previously quiescent genes. The kinetics and magnitude of changes in mRNA prevalence parallel changes in transcriptional activity; therefore the ethylene effect is primarily at the level of transcription. In vivo pulse labelling with [(35)S]-methionine showed that between 18 and 27 h of ethylene treatment a 2.5 fold increase in translational efficiency occurred for one message studied. The resulting protein is the predominant protein synthesized in carrots treated with ethylene for 27 h. Thus ethylene seemingly exerts multiple regulatory controls on the expression of genetic information.
植物激素乙烯在靶组织中引发许多生化变化。为了研究乙烯对遗传信息表达的影响，构建并分离了对应于乙烯诱导的胡萝卜根 mRNA 的 cDNA 克隆。RNA 点印迹分析表明，在所研究的三个克隆中，细胞质 mRNA 的峰值出现于处理后 21 小时，随后信使 RNA 迅速降解。DNA 滤膜过量杂交体外合成的核 RNA 表明，乙烯诱导的 mRNA 增加是由先前静止基因的转录引起的。mRNA 丰度变化的动力学和幅度与转录活性的变化平行；因此，乙烯的作用主要在转录水平上。用 [(35)S]- 蛋氨酸进行体内脉冲标记显示，在乙烯处理的 18 至 27 小时之间，所研究的一个 mRNA 的翻译效率增加了 2.5 倍。在乙烯处理 27 小时的胡萝卜中，该蛋白是合成的主要蛋白。因此，乙烯似乎对遗传信息的表达施加了多种调控控制。
