Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California 90024.
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4060-3. doi: 10.1073/pnas.79.13.4060.
The respiration of carrot (Daucus carota L.) roots is stimulated by ethylene. Polyribosomes were shown to proliferate concomitantly with the increase in respiration, and the extent of polyribosome augmentation was closely correlated to the amount of respiratory stimulation. In addition to the increase in quantity, ethylene caused a 2-fold increase in the average polyribosome size, suggesting tighter packing of ribosomes on RNA. In vitro translation of carrot polyadenylylated RNA with the rabbit reticulocyte lysate system followed by electrophoresis of the resulting translation products showed that ethylene treatment results in the appearance of new mRNAs.
乙烯能刺激胡萝卜(Daucus carota L.)根的呼吸。多核糖体与呼吸作用的增加同时增殖,多核糖体的扩增程度与呼吸刺激的程度密切相关。除了数量的增加外,乙烯还使多核糖体的平均大小增加了 2 倍,这表明 RNA 上的核糖体排列更加紧密。用兔网织红细胞裂解物系统体外翻译胡萝卜多聚腺苷酸化 RNA,然后对翻译产物进行电泳,结果表明乙烯处理会产生新的 mRNA。