Department of Horticulture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Plant Physiol. 1990 Jan;92(1):54-60. doi: 10.1104/pp.92.1.54.
Exogenous [(14)C]indole-3-acetic acid (IAA) is conjugated in citrus (Citrus sinensis) leaf tissues to one major substance which has been identified as indole-3-acetylaspartic acid (IAAsp). Ethylene pretreatment enhanced the catabolism of [(14)C]IAA to indole-3-carboxylic acid (ICA), which accumulated as glucose esters (ICGIu). Increased formation of ICGIu by ethylene was accompanied by a concomitant decrease in IAAsp formation. IAAsp and ICGIu were identified by combined gas chromatography-mass spectrometry. Formation of ICGIu was dependent on the concentration of ethylene and the duration of the ethylene pretreatment. It is suggested that the catabolism of IAA to ICA may be one of the mechanisms by which ethylene reduces endogenous IAA levels.
外源 [(14)C]吲哚-3-乙酸（IAA）在柑橘（Citrus sinensis）叶片组织中与一种主要物质结合，该物质已被鉴定为吲哚-3-乙酰天冬氨酸（IAAsp）。乙烯预处理增强了 [(14)C]IAA 向吲哚-3-羧酸（ICA）的分解代谢，ICA 积累为葡萄糖酯（ICGIu）。乙烯诱导的 ICGIu 形成增加伴随着 IAAsp 形成的相应减少。IAAsp 和 ICGIu 通过气相色谱-质谱联用技术进行鉴定。ICGIu 的形成取决于乙烯的浓度和乙烯预处理的持续时间。因此，IAA 向 ICA 的分解代谢可能是乙烯降低内源 IAA 水平的机制之一。
