Horisberger M, Tacchini-Vonlanthen M
Histochemistry. 1983;77(1):37-50. doi: 10.1007/BF00496634.
The soybean trypsin inhibitor (SBTI, Kunitz type) was localized by immunofluorescence and, at the ultrastructural level, by the protein A gold method on thin sections of Glycine max (soybean) cv. Maple Arrow. SBTI was localized in cell walls, protein bodies, the cytoplasm between the lipid-containing spherosomes, and the nucleus of the cotyledon and embryonic axis. In the nucleus, SBTI was present in the chromatin deposit and the nucleolus. The intensity of marking by the gold method decreased in the cell wall from the center of the cotyledon to the periphery. In four-days-old seedlings marking intensity of cell wall was much reduced. No SBTI could be detected in the hypocotyl. In two lines lacking soybean agglutinin (Norredo, T-102) the location of SBTI was similar to that observed in Maple Arrow. In P.I. 196168, a line lacking SBTI, marking intensity of the organelles was reduced to a very low level. Although this study was not designed to discern the cellular function of SBTI, if any, it may establish criteria consistent with its role in soybean.
通过免疫荧光以及在超微结构水平上，利用蛋白A金标法对栽培大豆品种Maple Arrow的薄片进行研究，从而对大豆胰蛋白酶抑制剂（SBTI，库尼茨型）进行定位。SBTI定位于细胞壁、蛋白体、含脂质球状体之间的细胞质以及子叶和胚轴的细胞核中。在细胞核中，SBTI存在于染色质沉积物和核仁中。金标法标记的强度在子叶从中心到周边的细胞壁中逐渐降低。在四天龄的幼苗中，细胞壁的标记强度大大降低。在下胚轴中未检测到SBTI。在两个缺乏大豆凝集素的品系（Norredo、T - 102）中，SBTI的定位与在Maple Arrow中观察到的相似。在缺乏SBTI的品系P.I. 196168中，细胞器的标记强度降低到非常低的水平。尽管本研究并非旨在识别SBTI（如果有的话）的细胞功能，但它可能建立与其在大豆中的作用相一致的标准。
