Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164.
Plant Physiol. 1985 Jul;78(3):652-4. doi: 10.1104/pp.78.3.652.
4-Methylmorpholine N-oxide monohydrate (MMNO.H(2)O), a potent solvent for polysaccharides, is an effective vehicle for release of membrane-enclosed male gametophytes (sporoplasts) from spore walls. This release occurs in minutes when pollen (Lilium longiflorum Thunb.) is suspended in a melt of MMNO.H(2)O at 75 degrees C. Continued heating at 75 degrees C leads to distintegration of the exine ;shell' which coalesces into immiscible globules in the MMNO melt. These observations provide a general procedure for preparation of pollen sporoplasts and sporoplast outer membranes, and offer a new method for dissolving the sporopollenin component of the spore wall.
4-甲基吗啉 N-氧化物一水合物（MMNO.H(2)O）是一种多糖的有效溶剂，也是从孢子壁中释放出被膜包裹的雄性配子体（孢子原生质体）的有效载体。当花粉（百合）悬浮在 75°C 的 MMNO.H(2)O 熔体中时，这种释放会在几分钟内发生。继续在 75°C 下加热会导致外壁的解体；壳“在 MMNO 熔体中聚合成不混溶的液滴。这些观察结果为花粉孢子原生质体和孢子原生质体外膜的制备提供了一般程序，并提供了一种溶解孢子壁中孢子花粉素成分的新方法。
