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从白花丹花粉中分离精子细胞

Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.

作者信息

Russell S D

机构信息

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019.

出版信息

Plant Physiol. 1986 May;81(1):317-9. doi: 10.1104/pp.81.1.317.

DOI:10.1104/pp.81.1.317
PMID:16664799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1075328/
Abstract

Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 x 10(6) cells/milliliter. Such ;gametoplasts' remain intact for up to 24 hours according to Evan's blue exclusion and can be used for characterization or physiological manipulation.

摘要

在花期即将到来时，通过用20%的蔗糖进行渗透休克处理，从成熟花粉粒中释放出完整的白花丹精子细胞。使用临床离心机通过差速离心法，精子细胞产量可达75%，细胞浓度可达8.8×10⁶个/毫升。根据伊文思蓝排斥法，这种“配子体”可在长达24小时内保持完整，可用于特性鉴定或生理操作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/16ebcd9d7bcc/plntphys00601-0324-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/1645945c43cb/plntphys00601-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/2a246e11c5df/plntphys00601-0324-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/16ebcd9d7bcc/plntphys00601-0324-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/1645945c43cb/plntphys00601-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/2a246e11c5df/plntphys00601-0324-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f8/1075328/16ebcd9d7bcc/plntphys00601-0324-c.jpg

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本文引用的文献

1
Pollen sporoplasts: dissolution of pollen walls.花粉原生质体：花粉壁的溶解。
Plant Physiol. 1985 Jul;78(3):652-4. doi: 10.1104/pp.78.3.652.
2
Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm.蔓花生的优先受精：被子植物配子水平识别的超微结构证据。
Proc Natl Acad Sci U S A. 1985 Sep;82(18):6129-32. doi: 10.1073/pnas.82.18.6129.
3
Isolation of vegetative and generative nuclei from pollen tubes.从花粉管中分离营养核和生殖核。
MeioCapture：一种在小麦减数分裂前期亚阶段中对减数分裂中期细胞进行分期和分离的有效方法。
BMC Plant Biol. 2018 Nov 21;18(1):293. doi: 10.1186/s12870-018-1514-z.
4
Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice.类受体激酶RUPO与钾转运体相互作用以调控水稻花粉管生长和完整性
PLoS Genet. 2016 Jul 22;12(7):e1006085. doi: 10.1371/journal.pgen.1006085. eCollection 2016 Jul.
5
Methods to isolate a large amount of generative cells, sperm cells and vegetative nuclei from tomato pollen for "omics" analysis.从番茄花粉中分离大量生殖细胞、精细胞和营养核用于“组学”分析的方法。
Front Plant Sci. 2015 Jun 2;6:391. doi: 10.3389/fpls.2015.00391. eCollection 2015.
6
Isolation and purification of generative cells from fresh pollen of Vicia faba L.从蚕豆新鲜花粉中分离和纯化生殖细胞
Plant Cell Rep. 1988 Mar;7(2):107-10. doi: 10.1007/BF00270116.
7
Flow Cytometric Characteristics of Sperm Cells Isolated from Pollen of Zea mays L.流式细胞术分析玉米花粉中精子细胞的特征
Plant Physiol. 1992 May;99(1):54-9. doi: 10.1104/pp.99.1.54.
8
Isolation of Fixed and Viable Eggs, Central Cells, and Embryo Sacs from Ovules of Plumbago zeylanica.从 Plumbago zeylanica 的胚珠中分离固定和存活的卵、中央细胞和胚囊。
Plant Physiol. 1989 May;90(1):9-12. doi: 10.1104/pp.90.1.9.
9
Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.蓝雪花成熟花粉粒和雄配子体中蛋白质及多肽的二维电泳研究
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10
Isolation of Sperms from the Pollen Tubes of Flowering Plants during Fertilization.受精过程中开花植物花粉管内精子的分离
Plant Physiol. 1988 Jul;87(3):647-50. doi: 10.1104/pp.87.3.647.
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Applications of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst 33258 in conjunction with the callose-specific aniline blue fluorochrome.荧光染料在花粉生物学中的应用。II. DNA探针溴化乙锭和Hoechst 33258与胼胝质特异性苯胺蓝荧光染料联合使用。
Stain Technol. 1985 May;60(3):155-62. doi: 10.3109/10520298509113906.