从白花丹花粉中分离精子细胞
Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.
作者信息
Russell S D
机构信息
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019.
出版信息
Plant Physiol. 1986 May;81(1):317-9. doi: 10.1104/pp.81.1.317.
Abstract
Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 x 10(6) cells/milliliter. Such ;gametoplasts' remain intact for up to 24 hours according to Evan's blue exclusion and can be used for characterization or physiological manipulation.
摘要
在花期即将到来时，通过用20%的蔗糖进行渗透休克处理，从成熟花粉粒中释放出完整的白花丹精子细胞。使用临床离心机通过差速离心法，精子细胞产量可达75%，细胞浓度可达8.8×10⁶个/毫升。根据伊文思蓝排斥法，这种“配子体”可在长达24小时内保持完整，可用于特性鉴定或生理操作。
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