Lin J J, Dickinson D B, Ho T H
Department of Horticulture, University of Illinois, 1301 W. Gregory Dr., Urbana, Illinois 61801.
Plant Physiol. 1987 Feb;83(2):408-13. doi: 10.1104/pp.83.2.408.
The accumulation of phytic acid during development of lily (Lilium longiflorum Thunb.) pollen and its degradation during germination have been studied. A substantial amount of phytic acid accumulates in lily pollen by 5 days before anthesis, and little change occurs during subsequent maturation. Mature lily pollen contains 7 to 8 micrograms phytic acid per milligram pollen. Considerable degradation of phytic acid occurs by 15 minutes of incubation in glucose culture medium, and very little is left by 3 hours. No partially phosphorylated myo-inositol accumulates during germination. The breakdown of phytic acid proceeds at a constant rate during this time period. The rate is calculated to be 0.037 microgram phytic acid/milligram pollen/minute. Two phytases are detected in germinated lily pollen extract using high performance liquid chromatography with an anion exchange column (diethylaminoethyl-5PW). The results suggest that one of the phytases is already present in mature ungerminated lily pollen and the other one is newly synthesized during germination from a long-lived, pre-existing mRNA.
研究了麝香百合（Lilium longiflorum Thunb.）花粉发育过程中植酸的积累及其萌发过程中的降解情况。在花期前5天，麝香百合花粉中积累了大量植酸，随后成熟过程中变化不大。成熟的麝香百合花粉每毫克含有7至8微克植酸。在葡萄糖培养基中孵育15分钟后，植酸发生显著降解，3小时后所剩无几。萌发过程中没有积累部分磷酸化的肌醇。在此时间段内，植酸的分解以恒定速率进行。计算得出速率为0.037微克植酸/毫克花粉/分钟。使用带有阴离子交换柱（二乙氨基乙基-5PW）的高效液相色谱法，在萌发的麝香百合花粉提取物中检测到两种植酸酶。结果表明，其中一种植酸酶已存在于未萌发的成熟麝香百合花粉中，另一种是在萌发过程中从长寿的、预先存在的mRNA中新合成的。
