Department of Biochemistry, University of Queensland, St. Lucia, Queensland 4067, Australia.
Plant Physiol. 1985 Aug;78(4):891-4. doi: 10.1104/pp.78.4.891.
Cloned hybridization probes have been used to investigate the effect of infection of susceptible Hordeum vulgare cv Prior by Erysiphe graminis f. sp. hordei on the abundance of host mRNAs coding for the large (LSU) and precursor to the small (SSU) subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase and the rapidly metabolized 32kD thylakoid protein (32kDP). In leaf RNA preparations from control (noninoculated) plants the amount of mRNA for the LSU and SSU declined from 7 to 11 days after sowing, whereas that for the 32kDP increased over this period. Following inoculation at 6 days after sowing, the abundance of each of the mRNA species was significantly reduced below that of controls at 1, 3, and 5 days later. Results indicate that infection causes a rapid and extensive reduction in host mRNA species coding for proteins with important photosynthetic functions.
已使用克隆杂交探针来研究易感染的大麦 cv. Prior 被禾柄锈菌 f. sp. hordei 感染对编码核酮糖 1,5-二磷酸羧化酶/加氧酶大亚基（LSU）和小亚基（SSU）前体以及快速代谢的 32kD 类囊体蛋白（32kDP）的宿主 mRNA 丰度的影响。在来自对照（未接种）植物的叶片 RNA 制剂中，LSU 和 SSU 的 mRNA 量从播种后 7 天到 11 天减少，而 32kDP 的 mRNA 量在此期间增加。在播种后 6 天接种后，每种 mRNA 物种的丰度在 1、3 和 5 天后均明显低于对照。结果表明，感染导致编码具有重要光合作用功能的蛋白质的宿主 mRNA 物种迅速而广泛减少。
