CSIRO Division of Tropical Crops and Pastures, CSIRO-UQ Plant Pathology Unit, Department of Botany, University of Queensland, 4067, St. Lucia, Australia.
Plant Cell Rep. 1987 Jun;6(3):204-7. doi: 10.1007/BF00268480.
Tumours were incited on leaf sections of Stylosanthes humilis, S. hamata, S. guianensis and S. scalra following infection by Agrobacterium tumefaciens. The suitability of 2 binary vectors (pGA472, BIN6) for gene transfer in S. humilis was tested and kanamycin-resistant tumour tissue was obtained from infected leaf pieces. The presence and expression of the neomycin phosphotransferase (NPT II) gene in the plant cells was demonstrated by hybridization of the coding region of the NPT II gene of the transposon Tn5 to DNA and RNA of kanamycin resistant tumours and by detection of significant NPT II activity in tissue extracts. Tumours also produced teratomatous shoots expressing the NPT II gene, but these could not be rooted.
在感染根癌农杆菌后，在柱花草、高山柱花草、圭亚那柱花草和卷茎蓼豆的叶片上引发了肿瘤。我们测试了 2 种二元载体（pGA472、BIN6）在柱花草中的基因转移适用性，并从感染的叶片中获得了卡那霉素抗性肿瘤组织。通过将转座子 Tn5 的 NPT II 基因的编码区与卡那霉素抗性肿瘤的 DNA 和 RNA 杂交，以及在组织提取物中检测到显著的 NPT II 活性，证明了植物细胞中 NPT II 基因的存在和表达。肿瘤还产生了表达 NPT II 基因的畸形芽，但这些芽无法生根。
