Reith M E, Cattolico R A
Department of Botany KB-15, University of Washington, Seattle, Washington 98195.
Plant Physiol. 1985 Sep;79(1):231-6. doi: 10.1104/pp.79.1.231.
This study represents the first report on chloroplast protein synthesis during the synchronous cell growth of a chromophytic (chlorophyll a,c) plant. When the unicellular alga Olisthodiscus luteus is maintained on a 12-hour light:12-hour dark cycle, cell and chloroplast number double every 24 hours. A temporal separation between these two events occurs. Measurements of chloroplast and total cellular protein values suggest that polypeptide synthesis occurs mainly in the light portion of the cell cycle, and pulse chase studies demonstrate that chloroplast proteins made in the light are not degraded in the dark. Data support the following conclusions: (a) a similar complement of chloroplast DNA coded proteins is made at all phases of the light portion of the cell cycle, and (b) chloroplast protein synthesis is a light rather than a cell cycle mediated response.
本研究是关于色素植物（叶绿素a、c）细胞同步生长过程中叶绿体蛋白质合成的首份报告。当单细胞藻类黄褐油球藻维持在12小时光照：12小时黑暗周期时，细胞和叶绿体数量每24小时翻倍。这两个事件之间存在时间间隔。叶绿体和总细胞蛋白质值的测量表明，多肽合成主要发生在细胞周期的光照阶段，脉冲追踪研究表明，在光照下合成的叶绿体蛋白质在黑暗中不会降解。数据支持以下结论：（a）在细胞周期光照阶段的所有时期，叶绿体DNA编码的蛋白质组成相似；（b）叶绿体蛋白质合成是对光照而非细胞周期的介导反应。
